Figure 8.

CD4⁺ T cell intrinsic galectin-1-deficiency promotes CD4⁺ T cell IFNγ production in the liver. Mixed bone marrow (BM) CD45.1/Lgals1^−/− (90:10) chimeras were generated as shown. Mice were infected with Leishmania donovani 3 months after BM engraftment (A). The frequencies of IFNγ-producing CD4⁺ T cells, Tr1 cells, and Treg cells in the liver were measured at day 14 p.i. (B). Treg cells were gated as described in Figure 1A. These data represent one of two separate experiments [n = 5 mice per group in each; **p < 0.01; Mann–Whitney test, wild-type (CD45.1) relative to Lgals1^−/− IFNγ⁺ CD4⁺ T cell frequency at day 14 p.i.].