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. 2017 Oct 16;7:13283. doi: 10.1038/s41598-017-13777-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Liver microenvironment related to liver damage. Association between: (a) Foxp3⁺ lymphocyte frequency and significant fibrosis, (b) IL-17A⁺ lymphocyte frequency and advanced fibrosis, (c) IL-17A⁺/Foxp3⁺ lymphocytes ratio and significant fibrosis (trend of association), (d) IL-17A⁺/Foxp3⁺ lymphocytes ratio and advance fibrosis, (e–f) IL-10 expression level with significant and advanced fibrosis, respectively, (g–h) TNF-α and TGF-β expression level with hepatitis severity, respectively. Significant (F ≥ 2) and advanced (F ≥ 3) fibrosis according to METAVIR. Hepatitis severity (min: minimal; mod: moderate, sev: severe) according to HAI. The results are depicted in box plots. Horizontal lines within boxes indicate medians. Horizontal lines outside the boxes represent the 5 and 95 percentiles. Mean is indicated as + . Frequencies were calculated as immunostained P-P/I lymphocytes/ total P-P/I lymphocytes in all portal tracts of the tissue section (400×). FC: fold change. The unpaired t-test (a,c,f,g and h) and Mann-Whitney U-test (b,d and e) were used to compare sets of data.