Fig. 1.

A) Schematic view of the hspX/esxS construct. HindIII and XhoI restriction sites were added at the 5’ and 3´ ends, respectively, the hspX and esxS genes are 435 and 294 bps in length, respectively, and the 15-amino acid flexible linker was 2x GGGGS. B) HspX/EsxS fusion protein profile. Amino acid composition; length, molecular weight, and tertiary structure are shown. The fusion protein tertiary structure was predicted by I-TASSER; the blue band (LYS1-THR145) is HspX, the green band (GLY159-GLU260) is EsxS, and the yellow band (THR145-GLY159) is the linker.