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. 2017 Oct 12;10:325. doi: 10.3389/fnmol.2017.00325

FIGURE 3.

FIGURE 3

Overview of the experimental protocol of in vitro and in vivo neurotransmitter release experiments. Ca2+-free Krebs’ solution (Ca-free) was perfused from 60 min before the start the sample collection in radiolabelled neurotransmitter release experiments, while TTX and CNQX-AP-5 was administered 15 min before first collected sample and until the end. Sumatriptan (SUMA), buspirone (BUSP), JNJ-47965567 (JNJ) and AZ-10606120 (AZ) were added the perfusion solution 15 min before second light stimuli and until the end. Exp, experiment; OS, optical stimulation; ES, electrical stimulation; SVER, chemical stimulation (veratridine); K+, chemical stimulation (KCl).