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. 2017 Oct 12;10:325. doi: 10.3389/fnmol.2017.00325

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Copyright © 2017 Gölöncsér, Baranyi, Balázsfi, Demeter, Haller, Freund, Zelena and Sperlágh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrical and optical stimulation results release of [³H]5-HT from superfused HP slices. (A) [³H] neurotransmitter release was measured from HP slices of virus-injected mice in response to different stimulations. The schematic figure illustrates the position of optical fiber. (B,C) [³H]5-HT release in response to electric (20 Hz) (B) and optogenetic (50 Hz) (C) stimulation. ES is an electrical stimulus, S_VER is a chemical (veratridine, 20 μM) stimulus, and OS is a light stimulation, n = 4–5 mice. (E,F) Frequency-dependence of [³H]5-HT release (10–100 Hz) from HP slices, n = 4-5 mice on each frequency. (H,I) Effects of TTX, CNQX-AP-5 and Ca²⁺-free conditions on the electrical (50 Hz) (H) and optical (50 Hz) (I) stimulation-evoked release of [³H]5-HT from HP. Ca²⁺-free Krebs’ solution was perfused from 60 min before the start the sample collection, while TTX and CNQX-DL-AP-5 was administered 15 min before first collected sample and until the end, n = 4 mice per group. (D,G) Sumatriptan, but not buspirone significantly decreased the electrically evoked release of [³H]5-HT in HP slices. [³H]5-HT release in response to optogenetic (50 Hz) (D) and electric (50 Hz) (G) stimulation, n = 4–5 mice per group. Sumatriptan and buspirone were added the perfusion solution 15 min before second light stimuli. OS₂ is a light stimulation and ES is an electrical stimulus. The tritium content in the perfusate samples (B,C) was expressed as fractional release (FR%) and is shown as a function of time. Curve shows the mean ± SEM of the identical experiments. Arrows indicate the time of different type of light, electric and chemical stimulations. (D–I) The results are expressed as the net release of tritium evoked by the first electrical or optical stimulation period (FRS₁, %). (D,G–I) Results were analyzed by one-way ANOVA, followed by Dunnett test, ^∗∗∗∗p < 0.0001, ^∗∗∗p < 0.001, ^∗p < 0.05. Asterisks indicate significant differences from control. (A) Created by modifying images purchased in the PPT Drawing Toolkits-BIOLOGY Bundle from Motifolio, Inc. (http://www.motifolio.com/neuroscience.html).