Table 1.
Comparison of uptake and release of [3H]5-HT and [3H]Glu in mice median raphe and hippocampus.
| Experimental group | [3H] uptake ( × 105 Bq/g) | Resting release (%) | ES1 Evoked release ( × 105 Bq/g) | SV ER Evoked release ( × 105 Bq/g) | Genotype |
|---|---|---|---|---|---|
| Experiment 1 | |||||
| 5-HT MR (n = 4) | 5.814 ± 2.217∗∗∗ | 0.86 ± 0.04 | 0.288 ± 0.153 | 1.155 ± 0.459 | WT |
| Glu MR (n = 3) | 58.532 ± 13.238 | 0.96 ± 0.01 | 1.059 ± 0.692 | 1.579 ± 0.593 | WT |
| 5-HT HP (n = 4) | 1.373 ± 0.182$$$ | 0.99 ± 0.09 | 0.045 ± 0.002 | 0.135 ± 0.019 | WT |
| Experimental group | [3H] uptake ( × 105 Bq/g) | Resting release (%) | OS1 Evoked release ( × 105 Bq/g) | ES Evoked release ( × 105 Bq/g) | Genotype |
| Experiment 2 | |||||
| 5-HT MR (n = 5) | 16.670 ± 2.888∗∗∗ | 1.83 ± 0.08### | 0.634 ± 0.083 | 1.242 ± 0.299∗ | WT |
| Glu MR (n = 8) | 51.425 ± 6.237 | 2.43 ± 0.18 | 0.622 ± 0.105 | 2.583 ± 0.334 | WT |
| 5-HT HP (n = 5) | 11.761 ± 1.939$$$ | 4.38 ± 0.33$$$ | 0.247 ± 0.085$ | 0.526 ± 0.096$$$ | WT |
| 5-HT HP (n = 5) | 13.617 ± 2.014$$$ | 4.58 ± 0.28$$$ | 0.172 ± 0.039#,$ | 0.264 ± 0.077$$$ | KO |
At 10 and 40 min after the start of the sample collection period tritium overflow was evoked by two identical periods of electrical (50 Hz, Experiment 1) and optical stimulation (50 Hz, Experiment 2), followed by a 3 min perfusion with the Na+ channel activator veratridine at 70 min (20 μM, SVER, Experiment 1) or 50 Hz electrical stimulation (Experiment 2). Means ± SEM of n observation are presented. The number of experiments is given in parenthesis. 5-HT MR vs. Glu MR ∗p < 0.05, ∗∗∗p < 0.001; 5-HT MR vs. 5-HT HP #p < 0.05, ###p < 0.001; 5-HT HP vs. Glu MR $p < 0.05, $$$p < 0.001, calculated by one-way ANOVA followed by the Tukey’s post hoc test.