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Chlorophyll a fluorescence over time of an axenic culture of the diatoms Leptocylindrus danicus (A–D) and Thalassiosira pseudonana (E–H) supplemented with 4-methyl-5-(β-hydroxyethyl)thiazole (HET; A,E), 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP; B,F), HET & HMP (C,G) and vitamin B1 (D,H) and a control without any of these supplements.
