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. 2017 Oct 16;7:13202. doi: 10.1038/s41598-017-13402-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Standard Curve for Detecting Human Genomic DNA among a Total of 100 ng of Mixed Human and Mouse Genomic DNA Using the Primer and Probe Set Devised in this Study (101 F, 206 R, and 144RH) (a) and that of McBride et al. (98 F, 188 R, and 133FH) (b). qPCR was performed for duplicate samples in one run, and the mean C_T values were plotted. For non-detected samples, the C_T values were assumed to be for 50 cycles. Negative control samples included TE buffer for the no-template control (NTC) and a sample of mouse genomic DNA only (Mo). Threshold cycles of detection were set at 2 cycles below the lower C_T value between NTC and Mo (red dotted lines), and the corresponding number of cycles is shown in red. The equations for linear approximations (blue lines) and the R² values are also shown.