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. 2017 Oct 16;7:13202. doi: 10.1038/s41598-017-13402-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of Human Cells in Mouse Xenotransplantation Models. (a) Detection of human cells in subrenal capsule assays. The indicated numbers of NHDF cells were injected into mouse subrenal capsules, and the numbers of cells calculated from the Alu-qPCR experiments are shown. qPCR was performed for triplicate samples in one run. Data represent the means, and bars indicate standard deviations for n = 3. (b) Detection of human cells in mouse organs at 1 day, 3 days, and 7 days after the injection of hMSCs into NOD/SCID mice via tail vein. qPCR was performed for triplicate samples in one run. The numbers of human cells calculated from the amount of human genomic DNA extracted from the lung, kidney, and liver are shown as box plots (n = 6). Whiskers represent minimum and maximum values, and the bold horizontal line represents the median. N.D. means not detected.