Table 1.
MegaBLAST search of the Alu primers and probe used in this study (left) and in the study by McBride et al. (right) against rodent genomes.
| Mouse | 20 nt | 19 nt | 18 nt | 20 nt | 19 nt | 18 nt | |
|---|---|---|---|---|---|---|---|
| 101 F | 0 | 0 | 0 | 98 F | 0 | 1 | 0 |
| 206 R | 0 | 0 | 1 | 188 R | — | 7 | 20 |
| 144RH | 0 | 0 | 1 | 133FH | 64 | 283 | 862 |
| Rat | |||||||
| 101 F | 0 | 1* | 0 | 98 F | 0 | 4 | 4 |
| 206 R | 0 | 0 | 0 | 188 R | — | 23 | 41 |
| 144RH | 0 | 0 | 0 | 133FH | 0 | 0 | 2 |
| Guinea pig | |||||||
| 101 F | 0 | 0 | 0 | 98 F | 0 | 1 | 0 |
| 206 R | 0 | 0 | 1 | 188 R | — | 91 | 316 |
| 144RH | 0 | 0 | 1 | 133FH | 1 | 36 | 91 |
The numbers of 100%-identity hits of 18–20 nt are shown. The lengths of 98 F and 188 R were 21 and 19 nt, respectively. The lengths of the other primers and the probe were 20 nt. *The 19-nt hit for 101 F against the rat genome might have been attributable to contamination by a human genomic sequence in the rat genome database, as described in the main text.