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. 2017 Oct 16;7:13201. doi: 10.1038/s41598-017-13501-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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csMVP is expressed on HCC cells but not on normal hepatocytes, and is induced under stressful environments, such as serum starvation, DNA damage, and detachment stress. (a) Flow cytometry analysis of HCC cells (Huh7, HepG2, and SNU-387), immortalized hepatocytes (THLE-2), NSCLC cells (A549 and H358), colorectal cancer cells (Colo205), PBMCs, and primary hepatocyte with α-MVP. (b) Immunocytochemical analysis of Huh7 cells with α-MVP and EGFR antibodies. Huh7 cells were incubated with α-MVP (red) and anti-EGFR (green) antibodies. Nuclei were labelled with DAPI. The scale bar is 20 μm. (c) Induced expression of csMVP on the surface of serum-starved Huh7 cells. Relative expression of csMVP was measured by mean fluorescence intensities (MFIs) of flow cytometric analysis of normal (con) or serum-starved Huh7 cells (starved). ***p < 0.005. (d) Induced expression of csMVP on the surface of etoposide-treated Huh7 cells. Relative expression of csMVP was measured by MFIs of flow cytometric analysis of DMSO or Etopo-treated Huh7 cells. *p < 0.05. (e) Induced expression of csMVP on Huh7 sphere cells. Relative expression of csMVP was measured by MFIs of flow cytometric analysis of attached or sphere cultures of Huh7 cells. ***p < 0.005.