Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 16;7:13294. doi: 10.1038/s41598-017-13444-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Biological effects induced by RANTES on EPC. (a) RANTES binds to EPC in a dose-dependent manner. Human EPC were incubated with 20 or 40 nM biotinylated RANTES (B-RANTES) and the binding were analysed by flow cytometer with avidin-FITC. Reactivity was compared to avidin-FITC alone. Data shown are representative of three independent experiments. (b) RANTES at 3 nM induced EPC spreading on fibronectin layer. Results are expressed as mean ± SEM of EPC area, expressed in square-inches, measured by field for three independent experiments. *P < 0.05 versus untreated cells. (c) RANTES at 3 nM induced EPC migration in a modified Boyden chamber model. Results are expressed as mean ± SEM of EPC counted by field for three independent experiments. *P < 0.05 versus untreated cells. (d) RANTES at 3 nM induces EPC vascular sprout area (left panel) and vascular sprout length (right panel) and in a 2D angiogenesis assay on Matrigel. The length and area of vascular sprouts formed by untreated EPC was arbitrary set to 100%. The results for cells treated with RANTES were expressed as a percentage of control cells. *P < 0.05 versus untreated cells.