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. 2017 Oct 10;21(2):351–365. doi: 10.1016/j.celrep.2017.09.038

Figure 1.

Figure 1

MC Response Profiles Become Sparse Following the Transition to Motherhood

(A) Confocal micrograph of labeled MCs in a Thy1-GCaMP3 mouse. GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cell layer; GCL, granule cell layer. Scale bar, 70 μm.

(B) In vivo two-photon micrographs of a Thy1-GCaMP3 mouse showing GCaMP3 labeling at −80, −180, and −280 μm from the dura. Scale bars, 70 μm.

(C) Two-photon micrograph of a representative field used for imaging in a naive female. Anes., anesthetized. Scale bar, 20 μm.

(D) Calcium transients elicited by the 12 neurons in the field shown in (C) in response to a 2-s odor stimulation with 6 monomolecular odors. Each trace represents a single trial. Black asterisks denote statistically significant responses. Vertical bar, 25% ΔF/F.

(E and F) As in (C) and (D), but data were collected from a mother.

(G) Cumulative distribution of peak odor-evoked responses in naive females (blue) and in mothers (red). Inset: mean ± SEM peak amplitude of odor-evoked calcium transients per cell in naive females (blue) and mothers (red) (∗p < 0.05, Mann-Whitney U test).

(H) Cumulative distribution of the percentages of MCs responding to 1–6 odors in naive females (blue) and in mothers (red). Each dashed line represents an individual mouse (∗∗∗p < 0.0001, Kolmogorov-Smirnov test).