Figure 4.

Similar Mixture Processing in Naive Females and Mothers

(A) Example of 2 MC responses to two odors and their mixtures (average of 4 trials). Top cell, mixture addition; bottom cell, mixture suppression. Etig, ethyl tiglate; Eac, ethyl acetate. Scale bars, 5 s, 25% ΔF/F.

(B) Left: MC responses from naive females to Etig (blue, sorted by amplitude) and the responses of the same MC to the mixture (black, Etig + Eac). Green circles correspond to the MC in (A). Right: MC responses to Etig and Eac + Etig mixture in mothers.

(C) Changes in ΔF/F (mean ± SEM) from odor responses to the mixture responses shown in (B). Green circles correspond to the MC in (A) and (B).

(D) Changes in ΔF/F (mean ± SEM) between all odor mixture combinations (see Experimental Procedures for details). p = 0.6, Mann-Whitney U test.

(E) Cumulative distribution of peak odor-evoked responses in naive females (blue) and in mothers (red) for the subset of mice used for the mixture experiments (n = 3 naive females, 3 mothers). Inset: mean ± SEM peak amplitude of odor-evoked calcium transients per cell in naive females (blue) and mothers (red) (^∗p < 0.05, Mann-Whitney U test).