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. 2017 Oct 16;7:13242. doi: 10.1038/s41598-017-12829-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Synthesis and functionalization of ⁶⁸Ga core-doped nanoradiotracers. (b) Hydrodynamic size (Z-average), determined by dynamic light scattering, of ten independent batches of citrate-coated NRT (blue dots); horizontal bars represent mean and 95%CI. (c) Hydrodynamic size (maximum peak in volume) of citrate-coated NRT in PBS, saline and mouse serum (from t = 0 to 24 hours). (d) PET images for different gallium concentrations in the ⁶⁸Ga-NRT. (e) ⁶⁸Ga-NRT hydrodynamic size, measured by dynamic light scattering, before and after conjugation of cFLFLF peptide. (f) TEM images of ⁶⁸Ga-NRT-cFLFLF at two magnifications (scale bars are 50 nm and 20 nm). (g) Comparison of FTIR spectra before and after conjugation of cFLFLF peptide. (h) Zeta potential before and after cFLFLF conjugation (statistical analysis by two-tailed t-test; error bars indicate s.d., N = 3; *, t 4.698 P 0.0182). (i) Thermogravimetric curve of ⁶⁸Ga-NRT-cFLFLF.