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. 2017 Oct 16;7:13242. doi: 10.1038/s41598-017-12829-y

Figure 3.

Figure 3

(a) PET/CT imaging of a C57BL/6 mouse, without LPS instillation, 1 h post i.v. injection with 68Ga-NRT-cFLFLF. (b) PET/CT imaging of a mouse with LPS-induced pulmonary inflammation (50 µg, tracheal instillation) 1 h post i.v. injection with 68Ga-NRT-cFLFLF. (c) PET/CT image of a neutrophil-depleted LPS-treated mouse 1 h post i.v. injection with 68Ga-NRT-cFLFLF. (d) Radiotracer organ biodistribution expressed as the percentage injected dose per gram (%ID/g) in LPS-instilled mice injected with 68Ga-NRT or 68Ga-NRT-cFLFLF (N = 5). (e) Percentage of in vivo labelled neutrophils measured by flow cytometry in LPS-instilled mice without nanoparticle injection (control) and 1 h post i.v. injection with fluorophore-conjugated 68Ga-NRT-cFLFLF. **, t 5.911, P 0.0038 by two-tailed t-test; error bars indicate s.d., N = 5, experiment repeated twice. (f) Radiotracer accumulation (%ID/g) in the lungs of LPS-instilled mice injected with 68Ga-NRT (black) or 68Ga-NRT-cFLFLF (white) and in the lungs of neutrophil-depleted LPS-instilled mice injected with 68Ga-NRT-cFLFLF (dark grey). ****P < 0.0001, n.s. P > 0.05, one-way ANOVA; error bars indicate s.d., N = 5, experiment repeated thrice. (g) Prussian blue staining of lung sections from an LPS-instilled mouse injected with 68Ga-NRT-cFLFLF (scale bars are 250 µm and 100 µm). (h) Prussian blue staining of lung sections from a neutrophil-depleted LPS-instilled mouse injected with 68Ga-NRT-cFLFLF (scale bars are 250 µm and 500 µm).