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. 2017 Oct 16;7:12813. doi: 10.1038/s41598-017-12648-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The roles of STIM1 and ORAI1 in LPS-induced COX-2 gene expression. STIM1 shRNA and ORAI1 shRNA were respectively transfected into AGS cells using Lipofectamine. (a) Immunoblot of STIM1 (80 kDa) and ORAI1 (31 kDa) after knockdown of the respective gene. 2 μg shRNA was used for transfection, and the total cell lysate was harvested for immunoblotting at 24 h post-transfection. (b) Cells were treated either with or without LPS after knockdown of STIM1 or ORAI1. RNA was extracted for COX-2 mRNA quantification by a real-time PCR. Statistical significance (*p < 0.05, **p < 0.01) of the difference was determined by Student’s t-test.