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. 2017 Oct 16;7:13297. doi: 10.1038/s41598-017-13567-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene and protein expression of collagens I and III in BMSC transfected with miR-29b mimic and inhibitor. (a) miR-29b (white bar), COL1A1 (light grey bar) and COL3A1 (dark grey bar) gene expression of BMSC in monolayer transfected with miR-29b mimic compared to BMSC transfected with a non-targeting control miR (NT-miR, zero line, n = 6). (b) Representative collagen I (n = 6) and III (n = 3) western blot images of total protein lysates of BMSC in monolayer transfected with mimic and NT-miR and corresponding β-Actin blots as loading controls. (c) miR-29b (white bar), COL1A1 (light grey bar), COL3A1 (dark grey bar) and COL2A1 (black bar) gene expression of BMSC transfected in monolayer with miR-29b inhibitor and subsequently cultured in micromass pellets for 7 days in chondrogenic medium conditioned with OA cartilage (CM) compared to BMSC transfected with NT-miR and cultured in CM (NT-miR + CM, zero line, n = 8). Results are expressed as box plots with median, the 25^th and 75^th percentiles and whiskers showing the largest and smallest values. * = p < 0.05; ** = p < 0.01; non-parametric Wilcoxon signed rank test for paired analysis. mimic: miR-29b mimic; NT-miR: non-targeting control miR; inhibitor + CM: with miR-29b inhibitor transfected BMSC cultured in medium conditioned with OA cartilage; NT-miR + CM: with non-targeting control miR transfected BMSC cultured in medium conditioned with OA cartilage.