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. 2017 Oct 16;7:13297. doi: 10.1038/s41598-017-13567-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of genes related to apoptosis in BMSC. (a) miR-29b (white bar), BCL2 (light grey bar) and MCL1 (dark grey bar) gene expression of BMSC kept in micromass pellets and cultured for 7 days in chondrogenic medium conditioned with OA cartilage (CM) compared to non-conditioned chondrogenic medium (NM, zero line, n = 7). (b) miR-29b (white bar), BCL2 (light grey bar) and MCL1 (dark grey bar) gene expression of BMSC in monolayer transfected with miR-29b mimic compared to BMSC transfected with a non-targeting control miR (NT-miR, zero line, n = 6). (c) miR-29b (white bar), BCL2 (light grey bar) and MCL1 (dark grey bar) gene expression of BMSC transfected in monolayer with miR-29b inhibitor and subsequently kept in micromass pellets and cultured for 7 days in CM compared to BMSC transfected with NT-miR and cultured in CM (NT-miR + CM, zero line, n = 8). Results are expressed as box plots with median, the 25^th and 75^th percentiles and whiskers showing the largest and smallest values. * = p < 0.05; ** = p < 0.01; non-parametric Wilcoxon signed rank test for paired analysis. CM: with OA cartilage conditioned medium; NM: non-conditioned medium; mimic: miR-29b mimic; NT-miR: non-targeting control miR; inhibitor + CM: with miR-29b inhibitor transfected BMSC cultured in medium conditioned with OA cartilage; NT-miR + CM: with non-targeting control miR transfected BMSC cultured in medium conditioned with OA cartilage.