Figure 1.

Resveratrol has a bacteriostatic effect on pre-grown NTHi cells. OD₆₀₀-normalized sBHI cultures of NTHi375 were divided into control untreated (circle); resveratrol 112.5 μg/ml (diamond); resveratrol 56.25 μg/ml (triangle); vehicle solution (square), and incubated for 4 h. At the indicated time points, OD₆₀₀ was recorded (a), and samples were serially diluted and plated on sBHI agar for c.f.u./ml determination (b). After 4 h, NTHi375 cells treated with both resveratrol 56.25 and 112.5 μg/ml showed a reduction in OD₆₀₀ and in the number of viable cells compared to control untreated (p < 0.0001) and vehicle solution cultures (p < 0.0001 and p < 0.001, respectively) cultures. Both OD₆₀₀ and number of viable cells were maintained through time in resveratrol treated cultures.