Figure 13. Influence of P79-98 on the transfection efficiency of HeLa by lipoplexes.

HeLa cells were transfected with 2.5-µg pCMV-eGFP. The plasmid was biotinylated (pDNA-bio) (biotin/DNA molar ratio of 3) and associated via streptavidin (STR) either with bio-P79-98 (P4) or bio-P38-57 (P1) as described in Figure 8a. The equipped plasmid was then complexed either with (white bar) KLN25/MM27 (DNA/lipid weight ratio of 1/2) or (black bar) Lipofectamine cationic liposomes. The fluorescence of cells was measured after 48-h transfection by flow cytometry and data were given as the percentage of transfected cells (% of positive cells), the mean of the fluorescence intensity (MFI) of the transfected cells and the global transfection efficiency (global TE) (i.e. the mathematical product of the MFI of cells and the percentage of fluorescent cells).