Figure 9. MT dependence.

HeLa cells were transfected with 2.5-µg pCMV-eGFP (5130 bp) encoding Yellow Fish enhanced GFP gene under the control of the CMV promoter. pDNA was biotinylated (pDNA-bio) (DNA/biotin molar ratio of 3) and associated either with bio-P79-98 (P4) or bio-P38-57 (P1) via streptavidin as described in Figure 8a. The equipped plasmid was then complexed with His–lPEI (DNA/polymer weight ratio of 1/6). The transfection was performed for 4 h at 37°C in the absence (grey bar) or the presence (black bar) of 33 µM nocodazole. Then the cells were washed and incubated for 48 h in fresh medium in the absence of nocodazole and any polyplexes. The fluorescence of cells was measured after 48 h transfection by flow cytometry. % stands for the percentage of transfected cells, MFI for the mean of the fluorescence intensity of the transfected cells and global transfection efficiency (TE) for the mathematical product of the MFI of cells and the percentage of fluorescent cells.