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. 2017 Oct 16;8:230. doi: 10.1186/s13287-017-0675-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Isl1⁺ CPCs remain viable and attach to the extracellular matrix of the patch material (SIS-ECM). a, b Isl1⁺ CPCs. c Extracellular matrix (SIS-ECM) patch material. d Scanning electron micrograph of SIS-ECM. e, f Viable Isl1⁺ CPCs prestained by Dil in SIS-ECM obtained by bright-field (e) and fluorescence (f) microscopy. g, h Images of frozen sections of Isl1⁺ CPCs seeded into SIS-ECM obtained by bright-field (g) and fluorescence microscopy (h). i Scanning electron microscopy shows attachment of Isl1⁺ CPCs (arrowhead) in SIS-ECM. j CPC proliferation rate in SIS-ECM compared to standard culture (control) measured using the Cell Counting Kit-8 (CCK-8) assay. SIS small intestinal submucosa