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. 2017 Oct 16;8:230. doi: 10.1186/s13287-017-0675-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Isl1⁺ CPCs differentiate into CMs and endothelial cells after seeding into SIS-ECM. a, c Isl1⁺ CPC-derived CMs (α-actinin-positive and cTnT-positive) in culture obtained by fluorescence microscopy. b, d Isl1⁺ CPC-derived CMs (α-actinin-positive and cTnT-positive) in SIS-ECM obtained by fluorescence microscopy. e Isl1⁺ CPC-derived endothelial cells (CD31⁺) in culture obtained by fluorescence microscopy. f Isl1⁺ CPC-derived endothelial cells (CD31⁺) in SIS-ECM obtained by fluorescence microscopy. g Relative quantitative RNA expression of α-actinin, cTnT, and CD31 by CPC-derived CMs or endothelial cells growing in SIS-ECM compared to standard culture plates. SIS small intestinal submucosa