Table 1.

Primer sequences used in this study

Primer name	Primer sequence (5′-3′)	Position on scaffold sequence	Annealing temperature (°C)	PCR product size	Function
Flo2-1F	TGTGCTGGAATCACCCACTC	793–812	60	1061	cloning TaFlo2 /polymorphism detection
Flo2-1R	GCGCGGCGAAAACTAATCAT	1853–1844
Flo2-2F	GTGCCGTCCATAATCGTTGC	1546–1565	60	1781	cloning TaFlo2 /polymorphism detection
Flo2-2R	CATGTGCGGCAAAAGACACA	3326–3307
Flo2-3F	AACGGGCATGTGTCTTTTGC	3299–3318	60	3025	cloning TaFlo2 /polymorphism detection
Flo2-3R	CGACGCAGCTCTGAAAATCG	6332–6313
Flo2-4F	CGCTTAGCAGTGGATTTGCC	5719–5738	60	3948	cloning TaFlo2
Flo2-4R	ATCCAACAAACAGGTGCCCA	9667–9647
Flo2-5F	TTGCGGAAGCCCATCATTCT	8387–8406	60	3836	cloning TaFlo2
Flo2-5R	TGACCTTCTGCGGATGCTTT	1222–12,203
Flo2-6F	CAGAACAGGGCCGGTACAAT	11,368–11,387	60	2600	cloning TaFlo2
Flo2-6R	CGCTCATCTGGATAGGGCAA	13,967–13,948
TaFlo2-InDel8F	ACCCCTCCTCCGTTATCGTC	1337–1356	60	145/153	8-bp InDel polymorphism in TaFlo2-A1
TaFlo2-InDel8R	CCTCCTTCTTCTTGCGGTCG	1470–1489
Flo2-A1F	GTGCTCCGATCCGATGTGCAGTTAT	5387–5411	58	587	2A specific
Flo2-A1R	GTGCACAACCAAGTAAAAGG	5973–5954
Flo2-B1F	GTC ATC ACTAGAGGA ATTTTCC	6851–6872	58	902	2B specific
Flo2-B1R	CTCTCAGAACTGTGGAT	7752–7736
Flo2-D1F	CTGTATCTGTAATTTGTTCCG	5378–5398	58	326	2D specific
Flo2-D1R	CTTCCGAAAAATGTGGGG	5704–5687
mFlo2-1F	TAACGGTGGTGCACTTGTGT	–	58	1868	Cloning mRNA
mFlo2-1R	TCAGCCGCAAGTTATGCTCA	–
mFlo2-2F	TGCGGACGAGATGGAAAACA	–	58	1809	Cloning mRNA
mFlo2-2R	AGCAGTCAGCCGATGGTATG	–
mFlo2-3F	ATGCGTACTCCCTAAGCGTG	–	58	1889	Cloning mRNA
mFlo2-3R	CACGAAGTGCTGCTTGCTTT	–
eTaFlo2F	CCATTCGGCTTTCGTGCAAA	–	55	134	Expression analysis
eTaFlo2R	TGTTTTCCATCTCGTCCGCA	–
ActinF	AGCCATACTGTGCCAATC	–	55	134	Internal control
ActinR	GCAGTGGTGGTGAAGGAGTAA	–