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. 2017 Oct 16;18:790. doi: 10.1186/s12864-017-4165-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Pilus and mucus binding of P. freudenreichii. a Pilus operons predicted by LOCP. b Transmission electron microscopy (TEM) images of the strains with intact operons (JS18 and JS20) and control (JS16). c Adhesion assay of specific binding to porcine mucus and non-specific binding to BSA with cell-free PBS as control. The difference in specific binding of strain JS18 to mucus compared to non-specific binding to BSA and background PBS was statistically significant (p < 0.05) and is marked with asterisk