Table 2.
Sequencing summary
| Sample Name | Sequencing Chemistry | SMRTcells | Movie Time (min) a | Total Number of Subreads | Total Number of Bases | Mean Subread Length (bp) | N50 Subread Length (bp) b |
|---|---|---|---|---|---|---|---|
| JS4 | P4/C2 | 2 | 120 | 142,306 | 465,129,578 | 3268 | 4131 |
| JS10 | P4/C2 | 2 | 120 | 205,113 | 606,802,054 | 2958 | 3733 |
| JS15 | P4/C2 | 2 | 120 | 179,642 | 540,206,310 | 3007 | 3653 |
| JS18 | P4/C2 | 2 | 120 | 194,246 | 550,199,736 | 2832 | 3679 |
| JS20 | P4/C2 | 2 | 120 | 230,371 | 632,635,668 | 2746 | 3456 |
| JS22 | P4/C2 | 2 | 120 | 181,361 | 563,541,807 | 4090 | 3107 |
| JS23 | P4/C2 | 2 | 120 | 192,373 | 617,310,336 | 3208 | 4222 |
| JS2 | P5/C3 | 2 | 240 | 100,864 | 719,516,424 | 7133 | 8940 |
| JS7 | P5/C3 | 2 | 240 | 107,194 | 1,002,978,417 | 9356 | 13,043 |
| JS8 | P5/C3 | 2 | 240 | 139,418 | 1,149,552,255 | 8245 | 11,148 |
| JS9 | P5/C3 | 2 | 240 | 160,808 | 1,316,084,462 | 8184 | 11,023 |
| JS11 | P5/C3 | 2 | 240 | 184,744 | 1,422,323,823 | 7698 | 10,498 |
| JS12 | P5/C3 | 2 | 240 | 207,391 | 1,490,421,212 | 7186 | 9765 |
| JS13 | P5/C3 | 2 | 240 | 103,466 | 793,823,369 | 7672 | 10,156 |
| JS14 | P5/C3 | 2 | 240 | 125,130 | 966,521,587 | 7724 | 10,326 |
| JS17 | P5/C3 | 2 | 240 | 151,002 | 1,149,295,071 | 7611 | 10,232 |
| JS21 | P5/C3 | 2 | 240 | 173,843 | 1,298,367,718 | 7468 | 9906 |
| JS25 | P5/C3 | 2 | 240 | 192,104 | 1,293,994,542 | 6735 | 8805 |
a run time during which nucleotides added by the polymerase are recorded in real-time
b minimum length of subreads in which half of the sequencing data is found