Fig. 7.

The effect of LIP treatment on cancer tissues and non-cancer tissues in vivo. a Mice with tumor growths were injected intratumorally (i.t.) 20 μg/kg rLIP or PBS. b-d Tumor growth of tumor-bearing nude mice were treated with either PBS or rLIP (n = 11 per group). Tumor size (b), tumor weight (c), body weight (d) of tumor-bearing nude mice. e Effect of LIP treatment on tumor tissues from xenografts. The cancer tissue pieces were treated with 2.5% glutaraldehyde for 24 h at 4 °C, and then a thin section of cancer tissues was observed via transmission electron microscopy (TEM). Red arrows, normal mitochondria; black arrows, mitochondria distension. f HE and IHC staining demonstrated that LIP induced the tumor cells death in vivo, as indicated by the expression of Ki67 and LIP, TUNEL-positive cells, and F4/80-positive cells. g Effect of LIP on non-cancer tissues from xenografts. Five different non-cancer tissues sections were stained by hematoxylin/eosin (H&E)