Skip to main content
. Author manuscript; available in PMC: 2017 Oct 17.
Published in final edited form as: Biomaterials. 2016 Jul 26;104:361–371. doi: 10.1016/j.biomaterials.2016.07.029

Figure 2.

Figure 2

In vitro characterization of the GO conjugates. (a) Western blotting of FSHR and β-actin in MDA-MB-231, cbgLuc-MDA-MB-231, and SKOV-3 cells. No observable change of FSHR was witnessed in MDA-MB-231 post transfection of cbgLuc. (b) Flow cytometry analysis of the GO conjugates in FSHR-positive MDA-MB-231 cells and FSHR-negative SKOV-3 cells. (c) Representative confocal fluorescence images of in MDA-MB-231 cells stained with fluorescein-GO-FSHR-mAb, fluorescein-GO and sonication-denatured fluorescein-GO-FSHR-mAb. Blank MDA-MB-231 cells were also used as a control. Scale bar: 50 μm. (d) Serum stability test of 64Cu-labeled GO conjugates after incubation in complete mouse serum for 24 h.