(A) Relative abundance of CsgA peptides with respect to the internal standard as a function of incubation time for peptide 1–25 , 26–37 , 38–48 , 49–69 , 70–83 , 84–93 and 106–137 . Data were fit with sigmoidal curves (dotted line). Error bars are standard deviation at each time point. (B) t1/2 value of each peptide extracted from sigmoidal curve fitting with errors indicating t1/2 ranges at 95% confidence level.