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. 2012 Jun 4;1(1):16. doi: 10.4102/ajlm.v1i1.16

FIGURE 1.

FIGURE 1

PCR amplification of the variable regions of class 1 and class 2 integrons. (a) Class 1 integron-variable regions amplified using Lev5’CS and Lev3’CS primers. Lane 1: No template; Lane 2: E. coli strain 042 bearing an aadA cassette within a class 1 integron; Lane 3: E. coli strain 17-2 bearing the dfrA1-sat-aadA cassette sequence within a class 2 integron; Lane 4: K. pneumoniae subsp. pneumoniae isolate K01 from this study; Lane 5: 1 kb Ladder plus (Invitrogen). Marker size fragments are indicated to the right of the gel in kilobase pairs. (b) Class 2 integron-variable regions amplified using hep51 and hep74 primers with samples shown in (a) loaded on to the gel. (c) Cassette content and orientation of the K01 integron amplified in (A), as predicted from the DNA sequence.