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. 2012 Jun 4;1(1):16. doi: 10.4102/ajlm.v1i1.16

TABLE 2.

Oligonucleotides for PCR reactions.

Target gene Primers
Amplicon size Reference
Name Sequence
Class 1 integron cassette region Lev5’CS 5’-GGC ATC CAA GCA GCA AG-3’ Varies with cassette content (0.7 Kb for aadA in control strain 042) 7 (Lévesque et al.)
Lev3’CS 5’ AAG CAG ACT TGA CCT GA-3’
Class 2 integron cassette region hep74 5′- CGG GAT CCC GGA CGG CAT GCA CGA TTT GTA- 3′ Varies with cassette content (2.2 Kb for dfrA1-sat1-aadA1 in control strain 17-2) 8 (White et al.)
hep51 5′-GAT GCC ATC GCA AGT ACG AG-3′
CTX-M genes CTX-MA 5′-CGC TTT G CG ATG TGC AG-3′ 0.55 Kb 10 (Bonnet et al.)
CTX-MB 5′-ACC GCG ATA TCG TTG GT-3′

Note: Please see the full reference list of the article, Aboderin AO, Adefehinti O, Odetoyin BW, Olotu AA, Okeke IN, Adeodu OO. Prolonged febrile illness due to CTX-M-15 extended-spectrum β-lactamase-producing Klebsiella pneumoniae infection in Nigeria. Afr J Lab Med. 2012;1(1), Art. #16, 4 pages. http://dx.doi.org/10.4102/ajlm.v1i1.16, for more information.