TABLE 2.
Target gene | Primers |
Amplicon size | Reference | |
---|---|---|---|---|
Name | Sequence | |||
Class 1 integron cassette region | Lev5’CS | 5’-GGC ATC CAA GCA GCA AG-3’ | Varies with cassette content (0.7 Kb for aadA in control strain 042) | 7 (Lévesque et al.) |
Lev3’CS | 5’ AAG CAG ACT TGA CCT GA-3’ | |||
Class 2 integron cassette region | hep74 | 5′- CGG GAT CCC GGA CGG CAT GCA CGA TTT GTA- 3′ | Varies with cassette content (2.2 Kb for dfrA1-sat1-aadA1 in control strain 17-2) | 8 (White et al.) |
hep51 | 5′-GAT GCC ATC GCA AGT ACG AG-3′ | |||
CTX-M genes | CTX-MA | 5′-CGC TTT G CG ATG TGC AG-3′ | 0.55 Kb | 10 (Bonnet et al.) |
CTX-MB | 5′-ACC GCG ATA TCG TTG GT-3′ |
Note: Please see the full reference list of the article, Aboderin AO, Adefehinti O, Odetoyin BW, Olotu AA, Okeke IN, Adeodu OO. Prolonged febrile illness due to CTX-M-15 extended-spectrum β-lactamase-producing Klebsiella pneumoniae infection in Nigeria. Afr J Lab Med. 2012;1(1), Art. #16, 4 pages. http://dx.doi.org/10.4102/ajlm.v1i1.16, for more information.