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. 2017 Oct 12;12:7501–7517. doi: 10.2147/IJN.S144623

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© 2017 Song et al. This work is published and licensed by Dove Medical Press Limited

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Induction of inflammasome response.

Notes: (A) Scheme of ex vivo experiment. (B, C) Secretion of IL-1β after incubation of BMDMs and BMDCs with various concentrations of aPNMs or carboxyl-terminated γ-PGA nanomicelles for 4 hours and after priming with LPS (400 ng mL⁻¹) for 3 hours. The concentration unit of the X-axis is µg mL⁻¹. ^***p<0.001. Scale bar is 15 µm. (1: control, 2: 1 µg mL⁻¹, 3: 2 µg mL⁻¹, 4: 5 µg mL⁻¹, 5: 10 µg mL⁻¹). Immunofluorescent images (100×) of the inflammasome complex (NLRP3/ASC) of BMDM (D) and BMDC (E).

Abbreviations: APC, antigen-presenting cells; aPNMs, amine-terminated γ-PGA nanomicelles; BMDCs, bone marrow-derived dendritic cells; BMDMs, bone marrow-derived macrophages; γ-PGA, poly-(γ-glutamic acid); LPS, lipopolysaccharide.

Induction of inflammasome response.

Notes: (A) Scheme of ex vivo experiment. (B, C) Secretion of IL-1β after incubation of BMDMs and BMDCs with various concentrations of aPNMs or carboxyl-terminated γ-PGA nanomicelles for 4 hours and after priming with LPS (400 ng mL⁻¹) for 3 hours. The concentration unit of the X-axis is µg mL⁻¹. ^***p<0.001. Scale bar is 15 µm. (1: control, 2: 1 µg mL⁻¹, 3: 2 µg mL⁻¹, 4: 5 µg mL⁻¹, 5: 10 µg mL⁻¹). Immunofluorescent images (100×) of the inflammasome complex (NLRP3/ASC) of BMDM (D) and BMDC (E).

Abbreviations: APC, antigen-presenting cells; aPNMs, amine-terminated γ-PGA nanomicelles; BMDCs, bone marrow-derived dendritic cells; BMDMs, bone marrow-derived macrophages; γ-PGA, poly-(γ-glutamic acid); LPS, lipopolysaccharide.