Figure 5.

AgNPs-induced apoptosis in F9 cells.

Notes: (A) F9 cells were treated with or without 12.5 μg/mL of AgNPs or 6.125 μM of AgNO₃ for 24 h. Then, the apoptosis of cells was assessed using the TUNEL assay; the nuclei were counterstained with DAPI. Representative images show apoptotic (fragmented) DNA (red staining) and the corresponding cell nuclei (blue staining). (B) Apoptotic efficiency was measured using flow cytometry.

Abbreviations: AgNPs, silver nanoparticles; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.