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. 2017 Oct 12;12:7529–7549. doi: 10.2147/IJN.S145147

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© 2017 Han et al. This work is published and licensed by Dove Medical Press Limited

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Effect of AgNPs on expression of differentiation markers.

Notes: (A) F9 cells were treated with or without RA (1 μM) for 72 h or AgNPs (6.125 or 12.5 μg/mL) for 24 h, and differentiation was observed by phase contrast microscopy. (B) Real-time quantitative PCR was performed to analyze the expression of various neuronal differentiation markers. The results are expressed as the mean ± standard deviation of three separate experiments. The treated groups showed statistically significant differences from the control group determined using Student’s t-test (***P<0.001).

Abbreviations: AgNPs, silver nanoparticles; RA, retinoic acid; PCR, polymerase chain reaction.