Table 1.
Primers used for real-time quantitative polymerase chain reaction for the analysis of differentiation and expression of apoptotic and antiapoptotic markers in stem cells
| Gene | Primer | TM (°C) |
|---|---|---|
| Nanog | F: CAGGCCTGGATGAAGAAGAG | 58 |
| R: ACTGGTAGAAGAATCAGGGCT | ||
| Rex1 | F: AAGCAGGATCGCCTCACTGT | 58 |
| R: GCTTCCAGAACCTGGCGAGA | ||
| p53 | F: AGAGACCGTACAGAAGA | 58 |
| R: CTGTAGCATGGGATCCTTT | ||
| p21 | F: GTTGCTGTCCGGACTACCG | 53 |
| R: AAAAACAATGCCACCACTCC | ||
| Caspase-3 | F: AGGGGTCATTTATGGGACA | 58 |
| R: TACACGGGATCTGTTTCTTTG | ||
| Caspase-9 | F: GTCACGGCTTTGATGGAGAT | 58 |
| R: CAGGCCTGGATGAAGAAGAG | ||
| Bax | F: CGAGCTGATCAGAACCATCA | 58 |
| R: GAAAAATGCCTTTCCCCTTC | ||
| Oct4 | F: CTCCCTACAGCAGATCACTCACA | 58 |
| R: AACCATACTCGAACCACATCCT | ||
| Bcl-2 | F: TAAGCTGTCACAGAGGGGCT | 58 |
| R: TGAAGAGTTCCTCCACCACC | ||
| RBP | F: GACAAGGCTCGTTTCTCTGG | 60 |
| R: AAAGGAGGCTACACCCCAGT | ||
| Laminin B1 | F: ACAACACCAAAGGCCTGAAC | 60 |
| R: TGCCAGTAGCCAGGAAGACT | ||
| Collagen type IV | F: AAAGGGAGAAAGAGGCTTGC | 60 |
| R: CCTTTGTACCGTTGCATCCT | ||
| GAPDH | F: AGGTCGGTGTGAACGGATTTG | 58 |
| R: TGTAGACCATGTAGTTGAGGTCA |
Abbreviation: TM, melting temperature.