Table 1.
Sequence of primers for real-time PCR
| Target | Sequence | Size (bp) | Tm (°C) | Reference |
|---|---|---|---|---|
| Total bacteria | ACTCCTACGGGAGGCAGCAGT ATTACCGCGGCTGCTGGC |
600 | 60 | 12 |
|
| ||||
| Firmicutes | ||||
| Clostridium leptum | GCACAAGCAGTGGAGT CTTCCTCCGTTTTGTCAA |
239 | 50 | 16 |
| Lactobacillus | TACATCCCAACTCCAGAACG AAGCAACAGTACCACGACC |
90 | 55 | 17 |
|
| ||||
| Bacteroidetes | ||||
| Bacteroides fragilis | ATAGCCTTTCGAAAGRAAGAT CCAGTATCAACTGCAATTTTA |
495 | 50 | 16 |
| Prevotella | CACRGTAAACGATGGATGCC GGTCGGGTTGCAGACC |
513 | 55 | 16 |
|
| ||||
| Bacteroidetes | ||||
| Bifidobacterium | CTCCTGGAAACGGGTGG GGTGTTCTTCCCGATATCTACA |
550 | 55 | 16 |
|
| ||||
| Protobacteria | ||||
| Escherichia coli | CATGCCGCGTGTATGAAGAA CGGGTAACGTCAATGAGCAAA |
95 | 55 | 18 |