Fig. 3.

PCR with the GlyF1 species-specific primer. The amplified products are obtained from single cyst samples of race 2, 4, 5, and X12, respectively. Lane M: DL2000; Lane 1: PCR amplification with universal primers D3A/D3B and approximately 345 bp fragments were yield from 28S gene of Heterodera glycines except NC; 2: PCR amplification with a species-specific primer GlyF1 and a universal primer DNA2 in combination and 181bp fragments were yield from ITS2 region and a part of the 28S gene of Heterodera glycines except NC. NC = negative control.