Figure 5. Rescue of clec-41 expression in adr-2(-) neural cells prevents disruptions in chemotaxis.
RNA was isolated from neural cells from wild-type (WT) as well as WT +clec-41 and adr-2(-)+clec-41 transgenic worms expressing clec-41 using the pan-neural rab-3 promoter for (A) qRT-PCR analysis of clec-41 expression. The endogenous control gpd-3 was used to normalize expression levels. (B) Chemotaxis assays used 60 cm plates. The chemoattractant (odorant) was spotted on one side and an ethanol control on the other. Worms were placed in the middle and allowed to migrate for 1 hr prior to counting (Wang et al., 2016) and the Chemotaxis Index of WT, adr-2(-) as well as WT +clec-41 and adr-2(-)+clec-41 to (C) Benzaldehdye (1:1000 dilution) or (D) Trimethylthiazole (1:10,000 dilution) was determined from 7 and 3 independent biological replicates, respectively. The chemotaxis index to trimethylthiazole (1:10,000 dilution) of worms expressing only the clec-41 3’ UTR in neural cells or expressing clec-41 in nonneural tissue was determined as a control (Figure 5—figure supplement 1). One-way ANOVA followed by Tukey’s Multiple Comparisons Correction. *p<0.05, **p<0.01.
Figure 5—figure supplement 1. Neural expression of clec-41 gene required for proper chemotaxis.
