Figure 3. 3D tracking of larval zebrafish.

(a) Representative time varying error signals in three dimensions, defined as the difference between real head position and set point. Inset provides magnified view at short time interval. Lateral movement can be rapidly compensated for within a few milliseconds with an instantaneous velocity of up to 10 mm/s. The axial shift was small compared with the depth coverage (200 μm) during whole brain imaging, and thereby had minor effect on brain activity reconstruction. (b) Tracking images at four time points during prey capture behavior, acquired at low (left) and high (right) magnification simultaneously. Scale bars are 1 mm (left) and 200 μm (right). (c) Kinematics of behavioral features during prey capture. Shaded region marks the beginning and end of the prey capture process.