Table 1.

Target genes, assays and forward, biotinylated reverse primers for bisulfite-specific PCR and sequencing primers for pyrosequencing

Target gene and assay	Forward primer	Reverse primer (biotinylated)	Sequencing primer
CYP27B1 Assay 1	GGTTTTTGGGGGTAGAGAAGAT	CTCCCTATTCCCAAACCCAATCAA	GGGGGTAGAGAAGATTTA
CYP27B1 Assay 2	AGAGGGGTTTGGGATGTT	AACCCTCAAATACCCCTCCAAAATATTCCAT	GGGATGTTTGTTAAGTT
VDR Assay 2	GGATTAGGGATTAGGGAAGTTGAGATTTA	TACTACTACAAAACCCCAAAAAACTCAACCTAA	AGATTTAGTTTTTTTGGGTGA
VDR Assay 3	ATTTTAATTTGTGGGATTAGGTTGAGT	TAATCCAAAATACAACCCCCCACCCTTCCTAC	TGGAGTTTTGTAGTAGTAATAGG
IGF2 Assay1	GGGATTGGGTTAGGAGAAGT	CCCCCCCAAAAATAACCAACAAT	GGGTTAGGAGAAGTTTTA
POMC Assay 7	GTTGGAAAGGGGTTGGAATTAGTA	ACACCCACAAAACCACTCCTAACTTCTAC	TTTAGGAAGAATTTAATTATGGAT

CYP27B1 = 1-alfa-hydroxylase gene, VDR = vitamin D receptor gene; IGF2 = insulin-like growth factor gene; POMC = proopiomelanocortin gene.