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. Author manuscript; available in PMC: 2017 Oct 17.
Published in final edited form as: J Alzheimers Dis. 2017;56(4):1263–1269. doi: 10.3233/JAD-161094

Figure 1.

Figure 1

Pen-2 together with presenilin could form a functionally active γ-secretase. (A) Upper panel, cell lysates were probed with antibody C15, which specifically recognize the last 15 amino acid of AβPP. Lower panel, immunoblot was probed with anti-GAPDH antibody as loading control. (B) Top panel, cell lysates were probed with anti-Myc antibody to detect the expression of NotchΔE. Middle panel, immunoblot was probed with anti-NICD (#4147), which specifically recognizes the N-terminus of NICD generated by γ-secretase activity. Bottom panel, immunoblot probed with anti-GAPDH antibody as loading control. (C) Knockdown of NCT in Aph-1-/- cell does not affect the Notch processing. Top panel, immunoblot was probed with anti-Myc antibody to detect the expression of NotchΔE. Second panel, immunoblot was probed with anti-NICD (#4147) to detect the formation of NICD. Third panel, immunoblot was probed with anti-NCT antibody to confirm the knockdown of NCT. Bottom panel, immunoblot probed with anti-GAPDH antibody as loading control.