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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cancer Res. 2017 Mar 30;15(7):929–941. doi: 10.1158/1541-7786.MCR-17-0023

Figure 3. Neurotensin and Insulin stimulate YAP/TEAD-regulated gene expression in PDAC.

Figure 3

Confluent PANC-1 cells (A) or MiaPaca-2 cells (B) were stimulated either without (−) or with 5 nM neurotensin (NT), 10 ng/ml insulin (Ins) or their combination (NT+Ins) for 30 or 60 min as indicated. RNA was isolated and the relative levels (n=3) of CTGF, CYR61 or CXCL5 mRNA compared with 18s mRNA was measured by RT-qPCR. Data are presented as mean ± SEM. Similar results were obtained in 3 independent experiments. NT + Ins significantly increased relative mRNA levels of CTGF, CYR61 or CXCL5 as compared with untreated controls (**P < 0.01) or with either NT or Ins (##P < 0.01; #P<0.05) as shown by one-way ANOVA with Bonferroni t-test.