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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Cell Microbiol. 2017 Jul 27;19(11):10.1111/cmi.12757. doi: 10.1111/cmi.12757

Figure 1.

Figure 1

EPEC induces internalization of Crb polarity proteins Crb3 and Pals1, but not Patj in intestinal epithelial cells. SKCO-15 cells were plated on Transwells and infected on the apical side, or not (UI), with EPEC for 2h. Immunolocalization of endogenous Crb3/Pals1/Patj and E-cad was performed. (A and B) Representative confocal images and quantification of the fluorescence intensity are shown. Arrows indicate the loss of membrane-associated Crb3 and Pals1 induced by EPEC. Hoechst was used to mark the nuclei (blue) in all the images. Scale bar, 10 μm. Data represent the mean ± SEM (n=3); ***P < 0.001 values were calculated using ANOVA Tukey’s Multiple Comparison Test.