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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Cell Microbiol. 2017 Jul 27;19(11):10.1111/cmi.12757. doi: 10.1111/cmi.12757

Figure 3.

Figure 3

Deletion of espF protects against Crb3 mislocalization while deletion of either map or espF attenuates the internalization of Pals1. SKCO-15 cells were plated on Transwells and infected apically with wild-type EPEC, ΔespF, Δmap, or complemented strains (ΔespF/pespF and Δmap/pmap) for 2h. Cells were immunostained for Crb3/Pals1/Patj and E-cad was used to label the lateral membrane. (A–F) Representative confocal images and quantification of the fluorescence intensity of membrane, cytoplasm, and total protein are shown. Arrows show the absence of membrane-associated Crb3 and Pals1 in infected monolayers. Scale bar, 10 μm. Data represent the mean ± SEM (n=3); ***P < 0.001 values were calculated using ANOVA Tukey’s Multiple Comparison Test.