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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Cell Microbiol. 2017 Jul 27;19(11):10.1111/cmi.12757. doi: 10.1111/cmi.12757

Figure 4.

Figure 4

Ectopic expression of EspF, but not Map, displaces Crb3 from the membrane. A) espF-HA or map-HA was cloned into the doxycycline-inducible pRetroX-Tight-Pur vector and transfected into SKCO-15 cells. Tet-On SKCO-15 cells were plated in absence of doxycycline (−dox) and transgene expression was induced with doxycycline (+dox) for 3 days. B) SKCO-15 cells were transiently transfected or not (control), with GFP-vector or GFP-EspF. Ectopic expression of EspF or Map was evaluated by immunodetection of HA (Green) or GFP (A and B, respectively). The impact of EspF or Map on localization of Crb3 and Patj was evaluated. Scale bar, 10 μm.