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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Cell Microbiol. 2017 Jul 27;19(11):10.1111/cmi.12757. doi: 10.1111/cmi.12757

Figure 5.

Figure 5

Depletion of espF or treatment with dynasore inhibits EPEC-induced cytoplasmic internalization of Crb3. (A–B) SKCO-15 cells were infected with wild-type EPEC, ΔespF or complemented strain (ΔespF/pespF) for 4h and processed for immunodetection of Crb3 and Rab5. (A and B) Representative confocal images and quantification of the area of the co-localization vesicles Crb3/Rab5 are shown. Data represent the mean ± SEM (n=3); ***P < 0.001 values were calculated using ANOVA Tukey’s Multiple Comparison Test. C) SKCO-15 cells were plated in Transwells and treated with DMSO or Dyn, then cells were infected or not (UI) with EPEC for 2h. Samples were processed for immunodetection of Crb3. Scale bar, 10 μm.