Fig 1. Analysis of the interaction of Plexin-B1-RBD and Plexin-B2-RBD with Rnd proteins.

ITC experiments were conducted with buffer containing 150 mM NaCl. Raw titration data showing the thermal effect and normalised heats of interaction of injecting (A): Plexin-B1-RBD into Rnd1, (B): Plexin-B1-RBD into Rnd2, (C): Plexin-B1-RBD into Rnd3, (D): Plexin-B2-RBD into Rnd1, (E): Plexin-B2-RBD into Rnd2 and (F): Plexin-B2-RBD into Rnd3. The experiments were conducted in 50 mM sodium phosphate pH 7.0, 150 mM NaCl, 4 mM MgCl₂ and 3 mM DTT at 25°C. The normalized heat of interaction was obtained in all cases by integrating the raw data and subtracting the heat of ligand dilution into the buffer alone. The grey line represents the best fit obtained by a non-linear least-squares procedure based on an independent binding sites model.