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. 2017 Oct 17;12(10):e0185899. doi: 10.1371/journal.pone.0185899

Fig 3. Analysis of the interaction of Plexin-B1-RBD and Plexin-B2-RBD with Rnd3 mutants.

Fig 3

ITC raw titration data showing the thermal effect and normalised heats of interaction of injecting (A): Plexin-B1-RBD into Rnd3Y60A, (B): Plexin-B1-RBD into Rnd3N86R, (C) Plexin-B1-RBD into Rnd3V87R, (D): Plexin-B1-RBD into Rnd3L90R, (E): Plexin-B1-RBD into Rnd3F124Y, (F): Plexin-B2-RBD into Rnd3Y60A, (G): Plexin-B2-RBD into Rnd3N86R (H): Plexin-B2-RBD into Rnd3V87R, (I): Plexin-B2-RBD into Rnd3L90R and (J): Plexin-B2-RBD into Rnd3F124Y. The experiments were conducted in 50 mM sodium phosphate pH 7.0, 150 mM NaCl, 4 mM MgCl2 and 3 mM DTT at 25°C. The normalized heat of interaction was obtained in all cases by integrating the raw data and subtracting the heat of ligand dilution into the buffer alone. The grey line represents the best fit obtained by a non-linear least-squares procedure based on an independent binding sites model.