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. 2017 Oct 17;12(10):e0186625. doi: 10.1371/journal.pone.0186625

Fig 1. Macropinocytosis does not require Ptpn22.

Fig 1

(A-E) Wild type (WT), Ptpn22-/- and Ptpn22R619W derived bone marrow derived dendritic cells (BMDC) were harvested and stimulated at 37°C for 0–30 minutes with Lucifer Yellow (LY). LY expression was determined by flow cytometry by gating on live, singlet, CD11c+ cells. (A) Representative flow cytometry plots showing (LY) uptake by WT (black solid line) and Ptpn22-/- (black dashed line), immature (top row) and LPS matured BMDC (bottom row). (B) LY uptake by WT (white square) and Ptpn22-/- (black square) immature BMDC. Data shown are Geometric Mean Fluorescent Intensity (GMFI) relative to maximal WT uptake at 30 minutes; N = 4 independent experiments; line represents mean ± s.d. (C) LY uptake by WT (white circle) and Ptpn22R619W (black circle) immature BMDC. Data shown are GMFI relative to maximal WT uptake at 30 minutes; N = 3 independent experiments; line represents mean ± s.d. (D) LY uptake by immature (-LPS) or LPS matured (+LPS) WT (white square) and Ptpn22-/- (black square) BMDC, showing representative LY GMFI of 3 independent experiments. (E) LY uptake relative to maximal WT uptake at 30 minutes by LPS matured WT (white square) and Ptpn22-/- (black square) BMDC. Data shown are GMFI mean ± s.d; N = 3 independent experiments.